Identification of serum proteins in dogs naturally infected with Anaplasma phagocytophilum and Borrelia burgdorferi: a pilot study

Serum proteomic analysis would aid in better understanding the pathophysiology of several diseases. The aim this study was to identify serum proteomes dogs with anaplasmosis and Lyme disease using a approach. Diseases were diagnosed by commercial rapid in-clinic ELISA. Borrelia antibodies evaluated IFAT. Four groups designated: symptomatic (n=5), dual-positive healthy control (n=5). samples collected before treatment. Two-dimensional electrophoresis pooled each group run triplicate. Ten out 57 differentially expressed spots between for identification mass spectrometry. Compared those controls, levels vitamin D-binding protein (VDBP), glycoprotein-9 (GP9) kininogen-1 (KGN-1) decreased, while haptoglobin (Hp) immunoglobulin (Ig) heavy chain increased dual infection group. apolipoprotein-A1 (Apo-A1) decreased anaplasmosis, infections compared dogs. clusterin but not or Calpain-3 disease. This showed that many might be changed naturally acquired Understanding role these proteins different biological processes can provide information interest diagnostic therapeutic approaches clinical conditions.